Reviewed, UniProtKB/Swiss-Prot Q9VVT2 (INDY1_DROME)

Names and origin

Protein names

Recommended name:
    Protein I'm not dead yet
Alternative name(s):
    INDY transporter protein
    drIndy

Gene names

Name:	Indy
ORF Names:	CG3979

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na(+), K(+), or Cl(-). Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines.
Subcellular location	Basolateral cell membrane; Multi-pass membrane protein. Note= Basolateral membrane of cells in the midgut.
Tissue specificity	In adults, abundantly expressed in the fat body, basolateral region of midgut cells and oenocytes. Low level expression is seen in the halteres, procardia, restricted regions of the esophagus and hindgut, base of the legs and in a subset of cells in the third segment of the antennae.
Induction	Completely inhibited by DIDS. Modest but significant inhibition by phloretin or furosemide.
Miscellaneous	The life-extending effect of mutations is likely caused by an alteration in energy balance caused by a decrease in transport function.
Sequence similarities	Belongs to the SLC13A transporter (TC 2.A.47) family. NADC subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	citrate transport Ref.1 Ref.7 Inferred from direct assay. Source: UniProtKB determination of adult life span Ref.6 Inferred from mutant phenotype. Source: FlyBase pyruvate transport Ref.1 Inferred from direct assay. Source: UniProtKB sodium ion transport Inferred from electronic annotation. Source: InterPro succinate transport Ref.1 Ref.7 Inferred from direct assay. Source: UniProtKB
Cellular component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Ref.7 Inferred from direct assay. Source: UniProtKB
Molecular function	citrate transmembrane transporter activity Ref.1 Ref.7 Inferred from direct assay. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct pyruvate transmembrane transporter activity Ref.1 Inferred from direct assay. Source: UniProtKB succinate transmembrane transporter activity Ref.1 Ref.7 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
spri	Q9W2U1	1	EBI-110127,EBI-99646

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VVT2-1)

Also known as: C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform B (identifier: Q9VVT2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Protein I'm not dead yet		PRO_0000172499
Regions
	Transmembrane	43 – 63	21	Potential
	Transmembrane	82 – 102	21	Potential
	Transmembrane	115 – 135	21	Potential
	Transmembrane	153 – 173	21	Potential
	Transmembrane	224 – 244	21	Potential
	Transmembrane	270 – 290	21	Potential
	Transmembrane	329 – 349	21	Potential
	Transmembrane	373 – 393	21	Potential
	Transmembrane	457 – 477	21	Potential
	Transmembrane	509 – 529	21	Potential
	Transmembrane	540 – 560	21	Potential
Natural variations
	Alternative sequence	1 – 18	18	Missing in isoform B.		VSP_029170

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (C) [UniParc]. Last modified November 13, 2007. Version 2. Checksum: ECA7857BB6B34414	FASTA	590	65,585
	10 20 30 40 50 60 MATETTKMIY TPPPLDIKME IEIGEQPQPP VKCSNFFANH WKGLVVFLVP LLCLPVMLLN 70 80 90 100 110 120 EGAEFRCMYL LLVMAIFWVT EALPLYVTSM IPIVAFPIMG IMSSDQTCRL YFKDTLVMFM 130 140 150 160 170 180 GGIMVALAVE YCNLHKRLAL RVIQIVGCSP RRLHFGLIMV TMFLSMWISN AACTAMMCPI 190 200 210 220 230 240 IQAVLEELQA QGVCKINHEP QYQIVGGNKK NNEDEPPYPT KITLCYYLGI AYASSLGGCG 250 260 270 280 290 300 TIIGTATNLT FKGIYEARFK NSTEQMDFPT FMFYSVPSML VYTLLTFVFL QWHFMGLWRP 310 320 330 340 350 360 KSKEAQEVQR GREGADVAKK VIDQRYKDLG PMSIHEIQVM ILFIFMVVMY FTRKPGIFLG 370 380 390 400 410 420 WADLLNSKDI RNSMPTIFVV VMCFMLPANY AFLRYCTRRG GPVPTGPTPS LITWKFIQTK 430 440 450 460 470 480 VPWGLVFLLG GGFALAEGSK QSGMAKLIGN ALIGLKVLPN SVLLLVVILV AVFLTAFSSN 490 500 510 520 530 540 VAIANIIIPV LAEMSLAIEI HPLYLILPAG LACSMAFHLP VSTPPNALVA GYANIRTKDM 550 560 570 580 590 AIAGIGPTII TIITLFVFCQ TWGLVVYPNL NSFPEWAQIY AAAALGNKTH
	Isoform B [UniParc]. Checksum: C021D77D006C5444		572	63,553
	10 20 30 40 50 60 MEIEIGEQPQ PPVKCSNFFA NHWKGLVVFL VPLLCLPVML LNEGAEFRCM YLLLVMAIFW 70 80 90 100 110 120 VTEALPLYVT SMIPIVAFPI MGIMSSDQTC RLYFKDTLVM FMGGIMVALA VEYCNLHKRL 130 140 150 160 170 180 ALRVIQIVGC SPRRLHFGLI MVTMFLSMWI SNAACTAMMC PIIQAVLEEL QAQGVCKINH 190 200 210 220 230 240 EPQYQIVGGN KKNNEDEPPY PTKITLCYYL GIAYASSLGG CGTIIGTATN LTFKGIYEAR 250 260 270 280 290 300 FKNSTEQMDF PTFMFYSVPS MLVYTLLTFV FLQWHFMGLW RPKSKEAQEV QRGREGADVA 310 320 330 340 350 360 KKVIDQRYKD LGPMSIHEIQ VMILFIFMVV MYFTRKPGIF LGWADLLNSK DIRNSMPTIF 370 380 390 400 410 420 VVVMCFMLPA NYAFLRYCTR RGGPVPTGPT PSLITWKFIQ TKVPWGLVFL LGGGFALAEG 430 440 450 460 470 480 SKQSGMAKLI GNALIGLKVL PNSVLLLVVI LVAVFLTAFS SNVAIANIII PVLAEMSLAI 490 500 510 520 530 540 EIHPLYLILP AGLACSMAFH LPVSTPPNAL VAGYANIRTK DMAIAGIGPT IITIITLFVF 550 560 570 CQTWGLVVYP NLNSFPEWAQ IYAAAALGNK TH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional identity of Drosophila melanogaster Indy as a cation-independent, electroneutral transporter for tricarboxylic acid-cycle intermediates." Inoue K., Fei Y.-J., Huang W., Zhuang L., Chen Z., Ganapathy V. Biochem. J. 367:313-319(2002) [PubMed: 12186628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[5]	"Large number of replacement polymorphisms in rapidly evolving genes of Drosophila. Implications for genome-wide surveys of DNA polymorphism." Schmid K.J., Nigro L., Aquadro C.F., Tautz D. Genetics 153:1717-1729(1999) [PubMed: 10581279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 366-572. Strain: Canton-S. Tissue: Embryo.
[6]	"Extended life-span conferred by cotransporter gene mutations in Drosophila." Rogina B., Reenan R.A., Nilsen S.P., Helfand S.L. Science 290:2137-2140(2000) [PubMed: 11118146] [Abstract] Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY.
[7]	"Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy." Knauf F., Rogina B., Jiang Z., Aronson P.S., Helfand S.L. Proc. Natl. Acad. Sci. U.S.A. 99:14315-14319(2002) [PubMed: 12391301] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF509505 mRNA. Translation: AAN86815.1. AE014296 Genomic DNA. Translation: AAF49226.1. AE014296 Genomic DNA. Translation: AAF49227.2. AE014296 Genomic DNA. Translation: AAN11670.1. AY102686 mRNA. Translation: AAM27515.1. AF217399 mRNA. Translation: AAF73384.1. Different initiation.
RefSeq	NP_524150.1. NP_730363.2. NP_730364.1.
UniGene	Dm.2528
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:18443N.
IntAct	Q9VVT2.
Genome annotation databases
Ensembl	CG3979. Drosophila melanogaster. [Contig view]
GeneID	40049.
KEGG	dme:Dmel_CG3979.
Organism-specific databases
FlyBase	FBgn0036816. Indy.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-017263-MON. DMEL-XXX-02:DMEL-XXX-02-017264-MON. DMEL-XXX-02:DMEL-XXX-02-017265-MON.
Gene expression databases
ArrayExpress	Q9VVT2.
GermOnline	CG3979. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001898. Na/sul_symport. [Graphical view]
Pfam	PF00939. Na_sulph_symp. 1 hit. [Graphical view]
PROSITE	PS01271. NA_SULFATE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	816740.

Entry information

Entry name

INDY1_DROME

Accession

Primary (citable) accession number: Q9VVT2
Secondary accession number(s): A4V236, Q7KUS6, Q9NHY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	November 13, 2007
Last modified:	November 25, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project