Reviewed, UniProtKB/Swiss-Prot Q5UR82 (SYM_MIMIV)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	MARS
Ordered Locus Names:	MIMI_R639

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Biophysicochemical properties	Kinetic parameters: KM=0.13 µM for tRNA-Met

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Methionyl-tRNA synthetase		PRO_0000139273
Regions
	Motif	10 – 22	13	"HIGH" region
	Motif	336 – 340	5	"KMSKS" region
Sites
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UR82-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 73B46C988F19465F	FASTA	550	63,682
	10 20 30 40 50 60 MQKFFVTSAL PYPNNSSPHL GNLVGALLSG DVYARFKRNQ GHEVIYLCGT DEYGTTTMIR 70 80 90 100 110 120 ARKEGVTCRE LCDKYFELHK KVYDWFNIEF DVFGRTSTTK QTEITWEIFN GLYNNGYIEE 130 140 150 160 170 180 KTTVQAFCEK CDMYLADTYL KGYCYHDGCR ENRVISNGDQ CEICQKMIDV NKLINPFCSI 190 200 210 220 230 240 CLTPPIQKST DHLYLSLDKL TPLVQQYLDR VEFDSRIMAI SKAWLEIGLN PRCITRDLEW 250 260 270 280 290 300 GTPIPINLDP KLEKYADKVF YVWFDAPIGY YSILANERDD WREWLNSGVT WVSTQAKDNV 310 320 330 340 350 360 PFHSIVFPAS VIGSNIELPL IDRICGTDYL LYEGQKFSKS QGVGLFGDKV AEISPKLGIN 370 380 390 400 410 420 EDYWRFYLMK IRPETQDSSF NLEEFVRIVK TDLVNNIGNF INRVFSLLEK TPYRDLNYQI 430 440 450 460 470 480 SPEYIEFIKK YEVSMDEFKF RDGLKICLEM SSRGNKFVQS TKPWTMIKDG LDTQEIMTEA 490 500 510 520 530 540 VGICWILLNL LKPIIPKSAC DMLSNLDTDN QNIFCLIGGS NINIRILNII KLPFKNIDLK 550 QLREFIEGKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.
[2]	"Virus-encoded aminoacyl-tRNA synthetases: structural and functional characterization of Mimivirus TyrRS and MetRS." Abergel C., Rudinger-Thirion J., Giege R., Claverie J.-M. J. Virol. 81:12406-12417(2007) [PubMed: 17855524] [Abstract] Cited for: CATALYTIC ACTIVITY, KINETIC PARAMETERS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653733 Genomic DNA. Translation: AAV50900.1.
RefSeq	YP_142993.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3161901.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

SYM_MIMIV

Accession

Primary (citable) accession number: Q5UR82

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	November 4, 2008
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)