Reviewed, UniProtKB/Swiss-Prot Q5UP36 (SYC_MIMIV)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	CARS
Ordered Locus Names:	MIMI_L164

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro cysteine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Cysteinyl-tRNA synthetase		PRO_0000159554
Regions
	Motif	21 – 31	11	"HIGH" region
	Motif	284 – 288	5	"KMSKS" region
Sites
	Metal binding	19	1	Zinc By similarity
	Metal binding	213	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Metal binding	242	1	Zinc By similarity
	Binding site	287	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UP36-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: EDEEC1AE9177AA0F	FASTA	509	59,332
	10 20 30 40 50 60 METELSETIL PTVTKMYVCG PTVYNDAHIG HARIYVIVDL INRTMNKILN KPTHLVMNVT 70 80 90 100 110 120 DIDDKIIRES KNKGITWLEL ARLHENSFFD CMSKLNVTRP DSVIRVTESI SDIVLYIQQI 130 140 150 160 170 180 INNGFAYIVS DSSVYFDSIE YKKAGYEFSE IDDEEEQQYE SLLSKEIVSQ KKHHKDFALW 190 200 210 220 230 240 KGRSESDVGF NVEFIFDNQT FKSFGVPGWH IECSAMIKKT LGNSIDIHFG GIDLKFPHHY 250 260 270 280 290 300 NECLQANAYH HPMYNPLHQS DTMIFHTWTR EFIHVGHLCI KGQKMSKSLK NFSTIKEMLD 310 320 330 340 350 360 KINSNQFRWL FMSTKWKQQV DFTDGLISIA KELDFTVVNF VNRVSNYPFE VSDVEFNDKE 370 380 390 400 410 420 TLLHDDFYRI QQRIYSYLTE FKFEMVARSI QHLIGTTNVY LDLPRPNESI VGKIRDYLLD 430 440 450 460 470 480 LLDKLGFIYR VGNSSSSHKI KDLMNILIET RSQLRQLTRN PDLSPGIKKQ LFDILDRQRN 490 500 IQLPDIGIIL EDSKDSSLWY ENSCVQSSE

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653733 Genomic DNA. Translation: AAV50438.1.
RefSeq	YP_142518.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3163027.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_MIMIV

Accession

Primary (citable) accession number: Q5UP36

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)