Reviewed, UniProtKB/Swiss-Prot Q38895 (SUP_ARATH)

Names and origin

Protein names

Recommended name:
    Transcriptional regulator SUPERMAN

Gene names

Name:	SUP
Synonyms:	FLO10
Ordered Locus Names:	At3g23130
ORF Names:	K14B15.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transcriptional regulator considered as cadastral protein that acts indirectly to prevent the B class homeotic proteins APETALA3 and perhaps PISTILLATA from acting in the gynoecial whorl. Principal function is to balance cell proliferation in the third and fourth whorls of developing flowers thereby maintaining the boundary between stamens and carpels. May fulfill this role by repressing genes implicated in cell division. Plays equally a role in the determinacy of the floral meristem. Is also required for normal ovule development.
Subcellular location	Nucleus.
Tissue specificity	Expressed early in the third whorl, and later becomes localized at the boundary between the stamen and carpel whorls. Also detected in developing ovule primordia.
Induction	Repressed by KRYPTONITE/SUVH4, member of the histone H3-K9 methyltransferase family that contributes with other factors to the CpNpG methylation of the SUP gene resulting in its silencing. The alternative epigenetic states of the SUPERMAN locus have been called Clark Kent alleles. Positively regulated at an early stage of development by LEAFY and by B class homeotic proteins APETALA3 and PISTILLATA. Later expression is maintained by both the B class homeotic proteins and the C class homeotic protein AGAMOUS. These two steps of regulation require the intervention of additional factors.
Domain	Contains two overlapping leucine-zipper-like motifs at the C-terminal region, which might serve as a site for protein-protein interaction. In this domain, a slightly degenerated ERF-associated amphiphilic repression (EAR) motif seems to be involved in the activity of transcriptional repression.
Miscellaneous	Mutations in SUP cause the ectopic expression of APETALA3 leading to the development of extra stamens at the expense of the central carpels of the flower.
Sequence similarities	Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Biological process	Differentiation Flowering Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Repressor
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 204

204

Transcriptional regulator SUPERMAN

PRO_0000047839

Regions

Domain

153 – 204

52

Leucine-zipper-like

Zinc finger

47 – 69

23

C2H2-type

Motif

195 – 199

5

EAR-like (transcriptional repression)

Experimental info

Mutagenesis

63

1

G → D in sup-3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

204

Helix Strand Turn

Details...

Beta strand

44 – 48

5

Turn

50 – 52

3

Beta strand

55 – 58

4

Helix

59 – 67

9

Turn

74 – 77

4

Sequences

	Sequence		Length	Mass (Da)
	Q38895-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2CF36E29A821F76E	FASTA	204	23,084
	10 20 30 40 50 60 MERSNSIELR NSFYGRARTS PWSYGDYDNC QQDHDYLLGF SWPPRSYTCS FCKREFRSAQ 70 80 90 100 110 120 ALGGHMNVHR RDRARLRLQQ SPSSSSTPSP PYPNPNYSYS TMANSPPPHH SPLTLFPTLS 130 140 150 160 170 180 PPSSPRYRAG LIRSLSPKSK HTPENACKTK KSSLLVEAGE ATRFTSKDAC KILRNDEIIS 190 200 LELEIGLINE SEQDLDLELR LGFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Role of SUPERMAN in maintaining Arabidopsis floral whorl boundaries." Sakai H., Medrano L.J., Meyerowitz E.M. Nature 378:199-203(1995) [PubMed: 7477325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTANT SUP-3. Strain: cv. Landsberg erecta.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The FLO10 gene product regulates the expression domain of homeotic genes AP3 and PI in Arabidopsis flowers." Schultz E.A., Pickett F.B., Haughn G.W. Plant Cell 3:1221-1237(1991) [PubMed: 12324589] [Abstract] Cited for: FUNCTION, MUTANT FLO-10. Strain: cv. Columbia.
[5]	"SUPERMAN, a regulator of floral homeotic genes in Arabidopsis." Bowman J.L., Sakai H., Jack T., Weigel D., Mayer U., Meyerowitz E.M. Development 114:599-615(1992) [PubMed: 1352237] [Abstract] Cited for: FUNCTION, MUTANT SUP-1. Strain: cv. Landsberg erecta.
[6]	"Regulation of SUP expression identifies multiple regulators involved in Arabidopsis floral meristem development." Sakai H., Krizek B.A., Jacobsen S.E., Meyerowitz E.M. Plant Cell 12:1607-1618(2000) [PubMed: 11006335] [Abstract] Cited for: FUNCTION, GENETIC REGULATION.
[7]	"The SUPERMAN protein is an active repressor whose carboxy-terminal repression domain is required for the development of normal flowers." Hiratsu K., Ohta M., Matsui K., Ohme-Takagi M. FEBS Lett. 514:351-354(2002) [PubMed: 11943180] [Abstract] Cited for: FUNCTION, IDENTIFICATION OF THE CARBOXY-TERMINAL REPRESSION DOMAIN.
[8]	"Control of CpNpG DNA methylation by the KRYPTONITE histone H3 methyltransferase." Jackson J.P., Lindroth A.M., Cao X., Jacobsen S.E. Nature 416:556-560(2002) [PubMed: 11898023] [Abstract] Cited for: INDUCTION.
[9]	"NMR structure of the single QALGGH zinc finger domain from the Arabidopsis thaliana SUPERMAN protein." Isernia C., Bucci E., Leone M., Zaccaro L., Di Lello P., Digilio G., Esposito S., Saviano M., Di Blasio B., Pedone C., Pedone P.V., Fattorusso R. ChemBioChem 4:171-180(2003) [PubMed: 12616630] [Abstract] Cited for: STRUCTURE BY NMR OF 41-79.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U38946 Genomic DNA. Translation: AAC49116.1.
AB025608 Genomic DNA. Translation: BAA95724.1.
DQ056605 mRNA. Translation: AAY78753.1.

PIR

S60325.

RefSeq

NP_188954.1.

UniGene

At.53448

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NJQ	NMR	-	A	42-78	[»]

ModBase

Search...

Genome annotation databases

GeneID

821888.

GenomeReviews

Gene locus AT3G23130 in contig BA000014_GR.

KEGG

ath:AT3G23130.

NMPDR

fig|3702.1.peg.14564.

Organism-specific databases

TAIR

At3g23130.

Gene expression databases

ArrayExpress

Q38895.

GermOnline

AT3G23130. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]

Pfam

PF00096. zf-C2H2. 1 hit.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 1 hit.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SUP_ARATH

Accession

Primary (citable) accession number: Q38895
Secondary accession number(s): Q4PSN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)