Reviewed, UniProtKB/Swiss-Prot P08409 (INSL_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative transposase insL for insertion sequence element IS186A/B/C

Gene names

Name:	insL1
Ordered Locus Names:	b0016, JW0015
AND
Name:	insL2
Ordered Locus Names:	b0582, JW0572
AND
Name:	insL3
Ordered Locus Names:	b2394, JW5390

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the transposition of the insertion sequence IS186.
Sequence similarities	Belongs to the transposase 11 family.

Ontologies

Keywords
Biological process	DNA recombination Transposition
Ligand	DNA-binding
Technical term	Complete proteome Transposable element
Gene Ontology (GO)
Biological process	transposition, DNA-mediated Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro transposase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Putative transposase insL for insertion sequence element IS186A/B/C		PRO_0000173294
Experimental info
	Sequence conflict	121	1	A → G in AAA25030. Ref.2
	Sequence conflict	126	1	S → T in AAA25030. Ref.2
	Sequence conflict	354 – 370	17	QPSLD…SEKKN → SHRWISPPEVPDPKRRTNSL WRITKMVIWSLQVAIRGTVS LTAYKTQLKNARHRLNEAPR RRILQMVQPLS in CAA26900. Ref.1
	Sequence conflict	354 – 370	17	QPSLD…SEKKN → SHRWISPPEVRIRKEELTRC GE in AAA25030. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08409-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: FDD2041F5F3D7F03	FASTA	370	40,909
	10 20 30 40 50 60 MNYSHDNWSA ILAHIGKPEE LDTSARNAGA LTRRREIRDA ATLLRLGLAY GPGGMSLREV 70 80 90 100 110 120 TAWAQLHDVA TLSDVALLKR LRNAADWFGI LAAQTLAVRA AVTGCTSGKR LRLVDGTAIS 130 140 150 160 170 180 APGGGSAEWR LHMGYDPHTC QFTDFELTDS RDAERLDRFA QTADEIRIAD RGFGSRPECI 190 200 210 220 230 240 RSLAFGEADY IVRVHWRGLR WLTAEGMRFD MMGFLRGLDC GKNGETTVMI GNSGNKKAGA 250 260 270 280 290 300 PFPARLIAVS LPPEKALISK TRLLSENRRK GRVVQAETLE AAGHVLLLTS LPEDEYSAEQ 310 320 330 340 350 360 VADCYRLRWQ IELAFKRLKS LLHLDALRAK EPELAKAWIF ANLLAAFLID DIIQPSLDFP 370 PRSAGSEKKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of a new GC-specific insertion element IS186." Chong P., Hui I., Loo T., Gillam S. FEBS Lett. 192:47-52(1985) [PubMed: 2996940] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"IS186: an Escherichia coli insertion element isolated from a cDNA library." Kothary R.K., Jones D., Candido E.P.M. J. Bacteriol. 164:957-959(1985) [PubMed: 2997142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[4]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (B2394). Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (B2394). Strain: K12 / MG1655 / ATCC 47076.
[7]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (B0016; B0582 AND B2394). Strain: K12 / MG1655 / ATCC 47076.
[8]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X03123 Genomic DNA. Translation: CAA26900.1. M11300 Genomic DNA. Translation: AAA25030.1. U82598 Genomic DNA. Translation: AAB40781.1. Different initiation. U00096 Genomic DNA. Translation: AAC73127.1. U00096 Genomic DNA. Translation: AAC73683.1. U00096 Genomic DNA. Translation: AAC75453.1. Different initiation. AP009048 Genomic DNA. Translation: BAA16264.1. Different initiation. AP009048 Genomic DNA. Translation: BAA35223.1. AP009048 Genomic DNA. Translation: BAE76030.1.
PIR	QQEC47. G65013.
RefSeq	AP_000680.1. AP_001228.1. AP_002993.1. NP_414557.1. NP_415114.1. NP_416895.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:28060N.
Genome annotation databases
GeneID	944754. 945200. 946896.
GenomeReviews	Gene locus b0016 in contig U00096_GR. Gene locus b0582 in contig U00096_GR. Gene locus b2394 in contig U00096_GR. Gene locus JW0015 in contig AP009048_GR. Gene locus JW0572 in contig AP009048_GR. Gene locus JW5390 in contig AP009048_GR.
KEGG	ecj:JW0015. ecj:JW0572. ecj:JW5390. eco:b0016. eco:b0582. eco:b2394.
Organism-specific databases
EcoGene	EG40012. insL.
CMR	Search...
Phylogenomic databases
HOGENOM	P08409.
Enzyme and pathway databases
BioCyc	EcoCyc:G6083-MON. EcoCyc:G7255-MON.
Family and domain databases
InterPro	IPR002559. Transposase_11. [Graphical view]
Pfam	PF01609. Transposase_11. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INSL_ECOLI

Accession

Primary (citable) accession number: P08409
Secondary accession number(s): P11307 , P76952, P77426, Q2MCH6, Q47051

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	July 15, 1998
Last modified:	November 25, 2008
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)