Reviewed, UniProtKB/Swiss-Prot O95445 (APOM_HUMAN)

Names and origin

Protein names

Recommended name:
    Apolipoprotein M
      Short name=Apo-M
      Short name=ApoM
Alternative name(s):
    Protein G3a

Gene names

Name:	APOM
Synonyms:	G3A, NG20
ORF Names:	HSPC336

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably involved in lipid transport.
Subcellular location	Secreted. Note= Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins (LDL).
Tissue specificity	Plasma protein. Expressed in liver and kidney.
Sequence similarities	Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	HDL Secreted
Domain	Signal
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	generation of precursor metabolites and energy Ref.1 Traceable author statement. Source: ProtInc membrane lipid metabolic process Ref.1 Traceable author statement. Source: ProtInc
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	lipid transporter activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Apolipoprotein M		PRO_0000223278
	Signal peptide	1 – ?22	22	Not cleaved
Amino acid modifications
	Glycosylation	135	1	N-linked (GlcNAc...)
	Disulfide bond	23 ↔ 167		Potential
	Disulfide bond	95 ↔ 183		Potential
	Disulfide bond	128 ↔ 157		Potential
Experimental info
	Mutagenesis	135	1	N → Q: Loss of glycosylation
	Mutagenesis	148	1	N → Q: No loss of glycosylation
	Sequence conflict	1 – 38	38	MFHQI…VDGKE → RFPDSIWGSRSDTSGSPQVP KLYFCGARRESPQPQT Ref.3
	Sequence conflict	175 – 188	14	LTPRN…ELSNN → VDS in CAB51604. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O95445-1 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: E4C35FEC32B7CB86	FASTA	188	21,253
	10 20 30 40 50 60 MFHQIWAALL YFYGIILNSI YQCPEHSQLT TLGVDGKEFP EVHLGQWYFI AGAAPTKEEL 70 80 90 100 110 120 ATFDPVDNIV FNMAAGSAPM QLHLRATIRM KDGLCVPRKW IYHLTEGSTD LRTEGRPDMK 130 140 150 160 170 180 TELFSSSCPG GIMLNETGQG YQRFLLYNRS PHPPEKCVEE FKSLTSCLDS KAFLLTPRNQ EACELSNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel human apolipoprotein (apoM)." Xu N., Dahlbaeck B. J. Biol. Chem. 274:31286-31290(1999) [PubMed: 10531326] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-15. Tissue: Liver.
[2]	"Characterisation of the novel gene G3a located in the class III region of the human major histocompatibility complex." Thomson W., Campbell R.D. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Human partial CDS from CD34+ stem cells." Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L., Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[4]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed: 14656967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region." Shiina S., Tamiya G., Oka A., Inoko H. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[8]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-135, MASS SPECTROMETRY. Tissue: Plasma.
[9]	"Proposed lipocalin fold for apolipoprotein M based on bioinformatics and site-directed mutagenesis." Duan J., Dahlbaeck B., Villoutreix B.O. FEBS Lett. 499:127-132(2001) [PubMed: 11418126] [Abstract] Cited for: 3D-STRUCTURE MODELING, MUTAGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF118393 mRNA. Translation: AAD11443.2. AJ245434 mRNA. Translation: CAB51604.1. AF161454 mRNA. Translation: AAF29014.1. AF129756 Genomic DNA. Translation: AAD18084.1. BA000025 Genomic DNA. Translation: BAB63389.1. AL662801 Genomic DNA. Translation: CAI18319.1. AL670886 Genomic DNA. Translation: CAI17788.1. AL805934 Genomic DNA. Translation: CAI18511.1. BC020683 mRNA. Translation: AAH20683.1.
RefSeq	NP_061974.2.
UniGene	Hs.534468
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O95445.
Proteomic databases
PeptideAtlas	O95445.
Genome annotation databases
Ensembl	ENSG00000204444. Homo sapiens. [Contig view] ENSG00000206304. Homo sapiens. [Contig view] ENSG00000206409. Homo sapiens. [Contig view]
GeneID	55937.
KEGG	hsa:55937.
Organism-specific databases
H-InvDB	HIX0005723. HIX0057956. HIX0058133.
HGNC	HGNC:13916. APOM.
MIM	606907. gene.
PharmGKB	PA38370.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O95445.
HOVERGEN	O95445.
Gene expression databases
ArrayExpress	O95445.
CleanEx	HS_APOM.
GermOnline	ENSG00000204444. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	61349.
SOURCE	Search...

Entry information

Entry name

APOM_HUMAN

Accession

Primary (citable) accession number: O95445
Secondary accession number(s): Q9P046, Q9UMP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 2000
Last modified:	November 25, 2008
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)