Reviewed, UniProtKB/Swiss-Prot O95365 (ZBT7A_HUMAN)

Names and origin

Protein names

Recommended name:
    Zinc finger and BTB domain-containing protein 7A
Alternative name(s):
    Leukemia/lymphoma-related factor
    Factor that binds to inducer of short transcripts protein 1
    Factor binding IST protein 1
      Short name=FBI-1
    HIV-1 1st-binding protein 1
    TTF-I-interacting peptide 21
      Short name=TIP21
    POZ and Krueppel erythroid myeloid ontogenic factor
      Short name=POK erythroid myeloid ontogenic factor
      Short name=Pokemon

Gene names

Name:	ZBTB7A
Synonyms:	FBI1, LRF, ZBTB7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals By similarity. Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' By similarity.
Subunit structure	Interacts with BCL6 By similarity.
Subcellular location	NucleusBy similarity.
Tissue specificity	Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level).
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 4 C2H2-type zinc fingers.
Sequence caution	The sequence AAB58414.1 differs from that shown. Reason: Frameshift at positions 8 and 324.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Repressor
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from sequence or structural similarity. Source: UniProtKB histone acetyltransferase binding Inferred from sequence or structural similarity. Source: UniProtKB transcription repressor activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 584

584

Zinc finger and BTB domain-containing protein 7A

PRO_0000047715

Regions

Domain

34 – 101

68

BTB

Zinc finger

382 – 404

23

C2H2-type 1

Zinc finger

410 – 432

23

C2H2-type 2

Zinc finger

438 – 460

23

C2H2-type 3

Zinc finger

466 – 490

25

C2H2-type 4; atypical

Compositional bias

175 – 211

37

Ala-rich

Compositional bias

175 – 181

7

Poly-Ala

Compositional bias

560 – 565

6

Poly-Gly

Amino acid modifications

Modified residue

337

1

Phosphoserine

Modified residue

341

1

Phosphoserine By similarity

Modified residue

526

1

Phosphoserine

Modified residue

549

1

Phosphoserine

Experimental info

Sequence conflict

554

1

G → C in AAH84568. Ref.3

Secondary structure

1

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584

Helix Strand Turn

Details...

Helix

16 – 29

14

Beta strand

36 – 40

5

Beta strand

43 – 47

5

Helix

49 – 55

7

Helix

57 – 64

8

Beta strand

73 – 76

4

Helix

82 – 94

13

Beta strand

95 – 97

3

Helix

101 – 103

3

Helix

104 – 113

10

Helix

117 – 127

11

Sequences

	Sequence		Length	Mass (Da)
	O95365-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 50073663F3D393A0	FASTA	584	61,439
	10 20 30 40 50 60 MAGGVDGPIG IPFPDHSSDI LSGLNEQRTQ GLLCDVVILV EGREFPTHRS VLAACSQYFK 70 80 90 100 110 120 KLFTSGAVVD QQNVYEIDFV SAEALTALMD FAYTATLTVS TANVGDILSA ARLLEIPAVS 130 140 150 160 170 180 HVCADLLDRQ ILAADAGADA GQLDLVDQID QRNLLRAKEY LEFFQSNPMN SLPPAAAAAA 190 200 210 220 230 240 ASFPWSAFGA SDDDLDATKE AVAAAVAAVA AGDCNGLDFY GPGPPAERPP TGDGDEGDSN 250 260 270 280 290 300 PGLWPERDED APTGGLFPPP VAPPAATQNG HYGRGGEEEA ASLSEAAPEP GDSPGFLSGA 310 320 330 340 350 360 AEGEDGDGPD VDGLAASTLL QQMMSSVGRA GAAAGDSDEE SRADDKGVMD YYLKYFSGAH 370 380 390 400 410 420 DGDVYPAWSQ KVEKKIRAKA FQKCPICEKV IQGAGKLPRH IRTHTGEKPY ECNICKVRFT 430 440 450 460 470 480 RQDKLKVHMR KHTGEKPYLC QQCGAAFAHN YDLKNHMRVH TGLRPYQCDS CCKTFVRSDH 490 500 510 520 530 540 LHRHLKKDGC NGVPSRRGRK PRVRGGAPDP SPGATATPGA PAQPSSPDAR RNGQEKHFKD 550 560 570 580 EDEDEDVASP DGLGRLNVAG AGGGGDSGGG PGAATDGNFT AGLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FBI-1, a factor that binds to the HIV-1 inducer of short transcripts (IST), is a POZ domain protein." Morrison D.J., Pendergrast P.S., Stavropoulos P., Colmenares S.U., Kobayashi R., Hernandez N. Nucleic Acids Res. 27:1251-1262(1999) [PubMed: 9973611] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Carcinoma.
[2]	"cDNA encoding TTF-I interacting peptide 21 (TIP21)." Jansa P., Grummt I. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Pancreas.
[4]	"Novel BTB/POZ domain zinc-finger protein, LRF, is a potential target of the LAZ-3/BCL-6 oncogene." Davies J.M., Hawe N., Kabarowski J., Huang Q.-H., Zhu J., Brand N.J., Leprince D., Dhordain P., Cook M., Moriss-Kay G., Zelent A. Oncogene 18:365-375(1999) [PubMed: 9927193] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Role of the proto-oncogene Pokemon in cellular transformation and ARF repression." Maeda T., Hobbs R.M., Merghoub T., Guernah I., Zelent A., Cordon-Cardo C., Teruya-Feldstein J., Pandolfi P.P. Nature 433:278-285(2005) [PubMed: 15662416] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-526, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337; SER-526 AND SER-549, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-526, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF097916 mRNA. Translation: AAC72973.1.
AF000561 mRNA. Translation: AAB58414.1. Frameshift.
BC084568 mRNA. Translation: AAH84568.1.
BC113511 mRNA. Translation: AAI13512.1.

RefSeq

NP_056982.1.

UniGene

Hs.591384

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IF5	X-ray	2.00	A	9-128	[»]
2NN2	X-ray	2.10	A/B	1-131	[»]

ModBase

Search...

PTM databases

PhosphoSite

O95365.

Genome annotation databases

Ensembl

ENSG00000178951. Homo sapiens. [Contig view]

GeneID

51341.

KEGG

hsa:51341.

Organism-specific databases

H-InvDB

HIX0014652.
HIX0092835.

HGNC

HGNC:18078. ZBTB7A.

MIM

605878. gene.

PharmGKB

PA134885165.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

O95365.

HOVERGEN

O95365.

Gene expression databases

ArrayExpress

O95365.

CleanEx

HS_ZBTB7A.

GermOnline

ENSG00000178951. Homo sapiens.

Family and domain databases

InterPro

IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

Gene3D

G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.

Pfam

PF00651. BTB. 1 hit.
PF00096. zf-C2H2. 3 hits.
[Graphical view]

ProDom

PD000003. Znf_C2H2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]

PROSITE

PS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

54786.

SOURCE

Search...

Entry information

Entry name

ZBT7A_HUMAN

Accession

Primary (citable) accession number: O95365
Secondary accession number(s): O00456, Q14D41, Q5XG86

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)