Reviewed, UniProtKB/Swiss-Prot O77459 (KEN_DROME)

Names and origin

Protein names

Recommended name:
    Transcription factor Ken
Alternative name(s):
    Protein Ken and Barbie

Gene names

Name:	ken
ORF Names:	CG5575

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor required for terminalia development. Negative regulator of the JAK/STAT pathway: represses JAK/STAT-dependent expression of ventral veins lacking (vvl) in the posterior spiracles.
Subcellular location	NucleusProbable.
Tissue specificity	Expressed from stage 5 in two rather faint stripes at positions of 64% (anterior domain; AD) and 17% (posterior domain; PD) egg length. During early gastrulation, at stage 6, these two stripes become more evident and detectable at the region posterior to the cephalic furrow and in the hindgut primordium. The AD disapears as gastrulation proceeds, while the PD remains. At stage 15, the AD appears again in the foregut, and PD expression in the hindgut and anal pad. In imaginal disks, it is ubiquitously expressed in both males and females in genital and eye-antennal disks. Not expressed in the brain. In genital disks, it is expressed along the margin of the anterior bulbus in males, while in females it is expressed in the posterior compartment along the anterior-posterior border, with medial expansion in the most posterior region.
Developmental stage	Expressed both maternally and zygotically. Highly expressed in embryos and pupae, and at lower level in larvae. In adults, it is expressed at higher level in females.
Miscellaneous	Defects in ken result in low mating success and reduced copulation duration. Men and female genitalia often remain inside the body, and genitalia and analia are missing in some homozygous flies. These flies are named after the famous dolls who also lack these 'features'.
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	female analia development Ref.3 Inferred from mutant phenotype. Source: UniProtKB female genitalia development Ref.3 Inferred from mutant phenotype. Source: UniProtKB insemination Inferred from mutant phenotype. Source: FlyBase male analia development Ref.3 Inferred from mutant phenotype. Source: UniProtKB male genitalia development Ref.3 Inferred from mutant phenotype. Source: UniProtKB negative regulation of JAK-STAT cascade Inferred from mutant phenotype. Source: UniProtKB phagocytosis, engulfment Inferred from mutant phenotype. Source: FlyBase regulation of transcription, DNA-dependent Ref.3 Ref.7 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	nucleus Ref.3 Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Ref.7 Inferred from mutant phenotype. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
lolal	Q7KRI2	1	EBI-193057,EBI-84493

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Transcription factor Ken		PRO_0000046925
Regions
	Domain	33 – 101	69	BTB
	Zinc finger	500 – 522	23	C2H2-type 1
	Zinc finger	528 – 551	24	C2H2-type 2
	Zinc finger	567 – 590	24	C2H2-type 3
	Compositional bias	245 – 258	14	His-rich
	Compositional bias	257 – 278	22	Asn-rich

Sequences

	Sequence		Length	Mass (Da)
	O77459-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C9AAA437DAD48FE8	FASTA	601	67,033
	10 20 30 40 50 60 MKEFQRMLML QYSKHGECIL KEIGAAFRGE HPADLTIVCE NKVKLHAHKL VLAAASPLIR 70 80 90 100 110 120 NLLEDTHLSD CSTTVYFPDV NATYFKFLLD FLYSGQTCIT SRDVNYLHDL LLLLQIKSDS 130 140 150 160 170 180 WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN 190 200 210 220 230 240 SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE IVNLSNAPPS GTSGSNSNIS 250 260 270 280 290 300 TSSNHQQQQH HHHHHHNHNN NNNNNNNNSS SSTINPVNLS LDLRTKSENS ASRTLGSGSD 310 320 330 340 350 360 HSGIDLAVTA SESTKRKGLF FDSHKDVMKP LSDGSDINSS PENYVVTPHR KRRPGFHNTQ 370 380 390 400 410 420 SDNQPFTSYP HSLLEELRLA KSTTSPISGF GSEKNMLAHL EDGALNGDTL TPDRKHLLEA 430 440 450 460 470 480 QRNRAQSPEI PMHLGPQFVY QWQSNQNAAM SAMPNLQSRL SSLSHISLNL DHPEGRSGSA 490 500 510 520 530 540 SGSGANLAGS NTHASSVREY RCEYCGKQFG MSWNLKTHLR VHTGEKPFAC RLCVAMFKQK 550 560 570 580 590 600 AHLLKHLCSV HRNVITTTNG ADTENRYSCC FCSMCFESVQ ELVRHLSGHH NNLLLTKNLR E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A transcription unit at the ken and barbie gene locus encodes a novel Drosophila zinc finger protein." Kuehnlein R.P., Chen C.-K., Schuh R. Mech. Dev. 79:161-164(1998) [PubMed: 10349629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"The Drosophila melanogaster 60A chromosomal division is extremely dense with functional genes: their sequences, genomic organization, and expression." Lukacsovich T., Asztalos Z., Juni N., Awano W., Yamamoto D. Genomics 57:43-56(1999) [PubMed: 10191082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"The ken and barbie gene encoding a putative transcription factor with a BTB domain and three zinc finger motifs functions in terminalia development of Drosophila." Lukacsovich T., Yuge K., Awano W., Asztalos Z., Kondo S., Juni N., Yamamoto D. Arch. Insect Biochem. Physiol. 54:77-94(2003) [PubMed: 14518006] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[7]	"Ken & barbie selectively regulates the expression of a subset of Jak/STAT pathway target genes." Arbouzova N.I., Bach E.A., Zeidler M.P. Curr. Biol. 16:80-88(2006) [PubMed: 16401426] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ012576 mRNA. Translation: CAA10062.1. AB010260 mRNA. Translation: BAA32683.1. AB010261 Genomic DNA. Translation: BAA32687.1. AE013599 Genomic DNA. Translation: AAF47084.1. AF181647 mRNA. Translation: AAD55433.1.
PIR	T00119.
RefSeq	NP_523833.1.
UniGene	Dm.2792
3D structure databases
HSSP	HSSP built from PDB template 7ZNF based on UniProtKB P08048.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:22018N.
IntAct	O77459.
Genome annotation databases
Ensembl	CG5575. Drosophila melanogaster. [Contig view]
GeneID	37785.
KEGG	dme:Dmel_CG5575.
NMPDR	fig|7227.3.peg.7156.
Organism-specific databases
FlyBase	FBgn0011236. ken.
Phylogenomic databases
HOGENOM	O77459.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-006318-MON.
Gene expression databases
ArrayExpress	O77459.
GermOnline	CG5575. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit. G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 1 hit.
Pfam	PF00651. BTB. 1 hit. PF00096. zf-C2H2. 2 hits. [Graphical view]
ProDom	PD000003. Znf_C2H2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	805393.

Entry information

Entry name

KEN_DROME

Accession

Primary (citable) accession number: O77459

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project